FCS file information

Input file name: Donor_Lung_032
Number of events: 738158

Quality control analysis

Summary

The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 95.03 %
Number of high quality events: 36686

Flow rate check

94.91 % anomalies detected in the flow rate check.

The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.

Signals acquisition check

0 % anomalies detected in the signal aquisition check.

The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.

The FCS file was divided in 1477 bins, hence each bin contains 500 event(s). The stable region is located between the bins 1 and 1477.

More info on the changepoints detected

No changepoints were detected.

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.

No changepoints were detected in the channels: 488 525/50-A, 488 715/30-A, 405 450/50-A, 405 515/20-A, 405 605/40-A, 405 670/30-A, 405 710/40-A, 405 780/60-A, 355 379/28-A, 355 515/30-A, 355 560/40-A, 355 740/35-A, 355 820/60-A, 640 670/30-A, 640 730/35-A, 640 780/60-A, 561 585/15-A, 561 610/20-A, 561 780/60-A, 405 585/15-A.

Dynamic range check

3.86 % anomalies detected in dynamic range check.

The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.

More info on the anomalies detected in the dynamic range check

For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:

##              lower_range upper_range
## 488 525/50-A        1374        5960
## 488 715/30-A        3050        2404
## 405 450/50-A         305        7850
## 405 515/20-A         278       17296
## 405 605/40-A         251        6712
## 405 670/30-A        1190        3212
## 405 710/40-A        2690        1804
## 405 780/60-A        4455          19
## 355 379/28-A        2084        4608
## 355 515/30-A         270        6417
## 355 560/40-A        2226        3855
## 355 740/35-A        2725         430
## 355 820/60-A        5287           9
## 640 670/30-A        2438          82
## 640 730/35-A        3718          22
## 640 780/60-A        3322         721
## 561 585/15-A        2107        2924
## 561 610/20-A        1900        1482
## 561 780/60-A        1765        1231
## 405 585/15-A         277        9640
## total_SUM          41712       76678
## total_UNIQUE        9176       19416

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.